Active Recombinant Human LRRN3, His-tagged

• Cat.No.: LRRN3-326H
• Product Overview: Recombinant Human LRRN3 (Accession # AAH35133) Val23-Thr628, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Val23
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to enhance the adhesion of Saos-2 human osteosarcoma cells to human Fibronectin.When 3 x 10^4 cells per well are added to rhNLRR-3 coated plates (5 µg/mL, 100 µL/well), approximately 50 - 70% will adhere after 1 hour at 37 °C.
• Molecular Mass: Recombinant Human LRRN3, His-tagged has a calculated MW of 69.3 kDa. In SDS-PAGE migrates as 90-95 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: LRRN3 leucine rich repeat neuronal 3 [ Homo sapiens ]
• Official Symbol: LRRN3
• Synonyms: LRRN3; leucine rich repeat neuronal 3; leucine-rich repeat neuronal protein 3; fibronectin type III; immunoglobulin and leucine rich repeat domains 5; FIGLER5; FLJ11129; NLRR3; neuronal leucine-rich repeat protein 3; leucine-rich repeat protein, neuronal 3; fibronectin type III, immunoglobulin and leucine rich repeat domains 5; NLRR-3
• Gene ID: 54674
• mRNA Refseq: NM_001099658
• Protein Refseq: NP_001093128
• UniProt ID: Q9H3W5
• Chromosome Location: 7q31.1