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Active Recombinant Human NID1 protein, His-tagged

Cat.No. : NID1-331H
Product Overview : Recombinant Human NID1(Leu29-Lys1114 (Gln1113Arg)) fused with His tag at N-terminal was expressed in NS0.
  • Specification
  • Gene Information
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Source : Mammalian cells
Species : Human
Tag : His
Predicted N Terminal : His
Form : Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity : Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. When 4 x 10^4 cells/well are added to rhNidogen-1 coated plates (30 µg/mL with 100 µL/well), approximately 40-75% will adhere after one hour at 37 centigrade.
Molecular Mass : Predicted Molecular Mass: 120 kDa;SDS-PAGE: 130 kDa, reducing conditions;
Endotoxin : <1.0 EU per 1 μg of the protein by the LAL method.
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Storage : Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : Reconstitute at 100 μg/mL in sterile PBS.
Gene Name : NID1 nidogen 1 [ Homo sapiens ]
Official Symbol : NID1
Synonyms : NID1; nidogen 1; NID, nidogen (enactin); nidogen-1; entactin; NID-1; enactin; NID;
Gene ID : 4811
mRNA Refseq : NM_002508
Protein Refseq : NP_002499
MIM : 131390
UniProt ID : P14543
Chromosome Location : 1q43
Function : calcium ion binding; collagen binding; extracellular matrix binding; laminin-1 binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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