Active Recombinant Bovine SPAM1, His-tagged

• Cat.No.: SPAM1-358B
• Product Overview: Recombinant Bovine SPAM1 (Accession # AAI10184) Met1-Thr497, fused with a C-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO cells
• Species: Bovine
• Tag: His
• Predicted N Terminal: Leu36
• Form: Supplied as a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to hydrolyze S-35 labeled bovine cartilage chondroitin sulfate.<20 ng of recombinant human SPAM1 is required for the 50% hydrolysis of 10 µg chondroitin sulfate, as measured under the described conditions.
• Molecular Mass: Recombinant Bovine SPAM1, His-tagged has a calculated MW of 53 kDa. In SDS-PAGE migrates as 60-70 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: SPAM1 sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) [ Bos taurus (cattle) ]
• Official Symbol: SPAM1
• Synonyms: SPAM1; sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding); PH-20; sperm adhesion molecule 1; hyaluronidase, zona pellucida binding; NP_001008413.3; EC 3.2.1.35
• Gene ID: 353352
• mRNA Refseq: NM_001008413
• Protein Refseq: NP_001008413
• Chromosome Location: 4