Recombinant Mouse Tlr9(Leu26-Asp818) fused with Fc tag at C-terminal was expressed in CHO.
Predicted N Terminal:
Lyophilized from a 0.2 μm filtered solution in PBS.
Measured by its ability to bind biotinylated CpG oligodeoxynucleotide, type B. The 50% maximum binding is achieved at 30‑150 ng/mL of Recombinant Mouse TLR9 Fc Chimera.Optimal dilutions should be determined by each laboratory for each application.
Predicted Molecular Mass: 115.8 (monomer) kDa;SDS-PAGE: 140-170 kDa, reducing conditions.
<0.10 EU per 1 μg of the protein by the LAL method.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitute at 100 μg/mL in PBS.