Active Recombinant Mouse Tnfsf18, His-tagged

• Cat.No.: Tnfsf18-408M
• Product Overview: Recombinant Mouse Tnfsf18 (Accession # AAO89011) Thr47-Ser173, fused with an N-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: His
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to stimulate mouse T cell proliferation in the presence of anti-CD3.The ED50 for this effect is typically 0.02-0.3 µg/mL in the presence of 10 µg/mL of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody.
• Molecular Mass: Recombinant Mouse Tnfsf18, His-tagged has a calculated MW of 16 kDa. In SDS-PAGE migrates as 16-26 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: Tnfsf18 tumor necrosis factor (ligand) superfamily, member 18 [ Mus musculus ]
• Official Symbol: Tnfsf18
• Synonyms: TNFSF18; tumor necrosis factor (ligand) superfamily, member 18; tumor necrosis factor ligand superfamily member 18; GITR ligand; glucocorticoid-induced TNF-related ligand; glucocorticoid-induced-tumor necrosis factor receptor ligand; Gitrl
• Gene ID: 240873
• mRNA Refseq: NM_183391
• Protein Refseq: NP_899247