Active Recombinant Human MADCAM1, Fc Chimera

• Cat.No.: MADCAM1-421H
• Product Overview: Recombinant Human MADCAM1 (Accession # AAY82472) was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: Fc Chimera
• Predicted N Terminal: Val23 & Glu29
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of HuT 78 human cutaneous T cell lymphoma cells.When 5 x 10^4 cells/well are added to recombinant human MAdCAM-1/Fc Chimera coated plates (2.5 µg/mL with 100 µL/well), approximately 50% - 70% will adhere after 1 hour incubation at 37 °C in the presence of 1 mM MnCl2.Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human MADCAM1, Fc Chimera has a calculated MW of 59.3 kDa (monomer). In SDS-PAGE migrates as 85-100 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: MADCAM1 mucosal vascular addressin cell adhesion molecule 1 [ Homo sapiens ]
• Official Symbol: MADCAM1
• Synonyms: MADCAM1; mucosal vascular addressin cell adhesion molecule 1; mucosal addressin cell adhesion molecule 1; MACAM1; MAdCAM-1; hMAdCAM-1; mucosal addressin cell adhesion molecule-1
• Gene ID: 8174
• mRNA Refseq: NM_130760
• Protein Refseq: NP_570116
• MIM: 102670
• UniProt ID: Q13477
• Chromosome Location: 19p13.3