Recombinant Human LY96(Glu17 - Asn160) fused with His tag at C-terminal was expressed in E. coli.
Predicted N Terminal:
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Measured by its binding ability in a functional ELISA. When recombinant human TLR-4 is Immobilized at 2 µg/mL (100 µL/well), the concentration of rhMD-2 that produces 50% optimal binding response is found to be approximately 0.03-0.15 µg/mL.
Predicted Molecular Mass: 19.2 (monomer) kDa
Glu17 - Asn160
<0.10 EU per 1 μg of the protein by the LAL method.
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.