Recombinant Human ACVR2B fused with Human IgG1 and His tag at C-terminal was expressed in insect cells.
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
The predicted molecular weight of Recombinant Human Act R-IIB is Mr 41 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 55-60 kDa.
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<1.0 EU/µg as determined by the LAL method
>95% by SDS-PAGE and analyzed by silver stain.
This lyophilized protein is stable for six to twelve months when stored desiccated at -20 centigrade to -70 centigrade. After aseptic reconstitution, this protein may be stored at 2 centigrade to 8 centigrade for one month or at -20 centigrade to -70 centigrade in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.