Active Recombinant Human BCAM, Fc Chimera

• Cat.No.: BCAM-502H
• Product Overview: Recombinant Human BCAM (Accession # CAA58449) was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: Fc Chimera
• Predicted N Terminal: Glu32
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of TE-85 human osteogenic sarcoma cells.When 5 x 10^4 cells/well are added to human BCAM/Fc coated plates (25 µg/mL with 100 µL/well), approximately 40 - 80% will adhere after 1 hour incubation at 37 °C.Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Human BCAM, Fc Chimera has a calculated MW of 83.7 kDa (monomer). In SDS-PAGE migrates as 110-120 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: BCAM basal cell adhesion molecule (Lutheran blood group) [ Homo sapiens ]
• Official Symbol: BCAM
• Synonyms: BCAM; basal cell adhesion molecule (Lutheran blood group); basal cell adhesion molecule (Lu and Au blood groups) , LU, Lutheran blood group (Auberger b antigen included); basal cell adhesion molecule; CD239; F8/G253 antigen; lutheran antigen; Auberger b antigen; glycoprotein 95kDa; B-cell adhesion molecule; B-CAM cell surface glycoprotein; lutheran blood group glycoprotein; antigen identified by monoclonal F8; basal cell adhesion molecule (Lu and Au blood groups); AU; LU; MSK19
• Gene ID: 4059
• mRNA Refseq: NM_001013257
• Protein Refseq: NP_001013275
• MIM: 612773
• UniProt ID: P50895
• Chromosome Location: 19q12-q13