Recombinant Human L1CAM (Accession # EAW63869) Ile25-Gln1096, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.
Predicted N Terminal:
Lyophilized from a 0.2 µm filtered solution in PBS.
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons.Able to significantly enhance neurite outgrowth when immobilized as a 3 µL droplet containing 100 ng on a nitrocellulose-coated microplate.
Recombinant Human L1CAM, His-tagged has a calculated MW of 121.3 kDa. In SDS-PAGE migrates as 160-180 kDa, reducing conditions.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).