Recombinant Human ITK protein(Arg352-Leu620), GST-tagged

• Cat.No.: ITK-576H
• Product Overview: Recombinant Human ITK (NP_005537.3) (Arg352-Leu620) was expressed in Insect Cells with a GST tag at the N-terminus.

Specification

• Source: Insect Cells
• Species: Human
• Tag: N-GST
• Protein length: Arg352-Leu620
• Form: Lyophilized from sterile 50 mM Tris, 150 mM NaCl, pH 7.5, 25 % glycerol, 0.5 mM PMSF, 0.5 mM TCEP, 0.1 mM EDTA, 0.5 mM GSH. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human ITK consists of 501 amino acids and predicts a molecular mass of 57.7 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 75 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ITK IL2-inducible T-cell kinase [ Homo sapiens ]
• Official Symbol: ITK
• Synonyms: ITK; IL2-inducible T-cell kinase; tyrosine-protein kinase ITK/TSK; EMT; LYK; PSCTK2; kinase EMT; T-cell-specific kinase; tyrosine-protein kinase LYK; IL-2-inducible T cell kinase; IL-2-inducible T-cell kinase; homolog of mouse T-cell itk/tsk; interleukin-2-inducible T cell kinase; interleukin-2-inducible T-cell kinase; MGC126257; MGC126258
• Gene ID: 3702
• mRNA Refseq: NM_005546
• Protein Refseq: NP_005537
• MIM: 186973
• UniProt ID: Q08881