Active Recombinant Human SPP1, His-tagged

• Cat.No.: SPP1-616H
• Product Overview: Recombinant Human SPP1 (Accession # NP_000573.1) Ile17-Asn300, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Ile17
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. Agnihotri, R. et al. (2001) J. Biol. Chem. 276:28261.Recombinant Human (rh) Osteopontin/OPN immobilized at 1 µg/mL, 100 µL/well, will mediate the adhesion of added 293 cells >30% (1 x 10^5 cells/well). Recombinant Human Coagulation Factor II/Thrombin proteolytic treatment of this rhOsteopontin/his can increase HEK293 cell adhesion by about 5-fold.
• Molecular Mass: Recombinant Human SPP1, His-tagged has a calculated MW of 32.9 kDa. In SDS-PAGE migrates as 60-65 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: PPP3CA protein phosphatase 3, catalytic subunit, alpha isozyme [ Homo sapiens ]
• Official Symbol: PPP3CA
• Synonyms: PPP3CA; protein phosphatase 3, catalytic subunit, alpha isozyme; CALN, CALNA, protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform , protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform (calcineurin A alpha); serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform; calcineurin A alpha; CNA1; PPP2B; protein phosphatase 2B; catalytic subunit; alpha isoform; CAM-PRP catalytic subunit; calmodulin-dependent calcineurin A subunit alpha isoform; protein phosphatase 2B, catalytic subunit, alpha isoform; protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform; CALN; CCN1; CALNA; CALNA1
• Gene ID: 5530
• mRNA Refseq: NM_000944
• Protein Refseq: NP_000935
• MIM: 114105
• UniProt ID: Q08209
• Chromosome Location: 4q24