Active Recombinant Human RIPK2, His-tagged

• Cat.No.: RIPK2-419H
• Product Overview: Recombinant Human RIPK2, Catalytic Domain (amino acids 1-299), fused with His-tagged, expressed in insect cells.

Specification

• Description: This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal caspase recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-κB and inducer of apoptosis in response to various stimuli.
• Source: Insect cells
• Species: Human
• Tag: His
• Form: 50 mM Tris (pH 7.5), 150 mM NaCl, 0.5 mM EDTA, 0.02% Triton® X–100, 2 mM DTT and 50% Glycerol.
• Bio-activity: 37 nmoles of phosphate transferred to myelin basic protein (MBP) per minute per mg of total protein at 30 centigrade. Activity determined at a final protein concentration of 16.7 μg/mL.
• Molecular Mass: 38.9 kDa. Calculated from the protein sequence(s)
• Protein length: amino acids 1-299
• Purity: 75% as determined by a Coomassie® blue stained SDS-PAGE gel.
• Storage: For maximum recovery please spin prior to use. Aliquots of the 5 ug, 10ug and 20ug sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at ?80 centigrade, this product is guaranteed for 6 months from date of purchase.
• Concentration: 0.47 mg/mL total protein as measured using the Bradford protein assay with BSA as a standard.
• Dilutions: 20 mM Tris (pH 7.5), 0.02% Triton® X?100, 0.1 mg/mL BSA, 2 mM DTT, 0.5 mM Na3VO4 and 10% Glycerol.
• Shipping: dry ice

Gene Information

• Gene Name: RIPK2 receptor-interacting serine-threonine kinase 2 [ Homo sapiens ]
• Official Symbol: RIPK2
• Synonyms: CCK; RICK; RIP2; CARD3; GIG30; CARDIAK; receptor-interacting serine/threonine-protein kinase 2; CARD-carrying kinase; CARD-containing IL-1 beta ICE-kinase; CARD-containing interleukin-1 beta-converting enzyme (ICE)-associated kinase; RIP-2; growth-inhibiting gene 30; receptor-interacting protein (RIP)-like interacting caspase-like apoptosis regulatory protein (CLARP) kinase; receptor-interacting protein 2; tyrosine-protein kinase RIPK2
• Gene ID: 8767
• mRNA Refseq: NM_003821
• Protein Refseq: NP_003812
• MIM: 603455
• UniProt ID: O43353
• Chromosome Location: 8q21
• Pathway: Activated TLR4 signalling, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Canonical NF-kappaB pathway, organism-specific biosystem
• Function: ATP binding; CARD domain binding; LIM domain binding