Active Recombinant Human FLT3LG

• Cat.No.: FLT3LG-1235H
• Product Overview: Recombinant Human Flt3-Ligand is a soluble 17.6kDa polypeptide containing 155 amino acid residues, which comprises of the extracellular transmembrane domain of the Flt3-ligand protein. Flt3-ligand (flk2-ligand) stimulates the proliferation and colony formation of certain BM precursor cells including CD34+ cells.

Specification

• Description: Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3-LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8(see MIM 186910)-positive classical DCs and their CD103-positive tissue counterparts.
• Source: E. coli
• Species: Human
• Form: Lyophilized from a 0.2μm filtered solution in 20mM Tris-HCl, 20mM NaCl, pH7.4.
• Bio-activity: rHuFlt3-L is fully biologically active when compared to standards. Its specific activity is .1.106 units/mg.
• Endotoxin: ≤1EU/μg, determined by the LAL method.
• Purity: ≥98%, as determined by SDS-PAGE and HPLC method.
• Usage: FOR RESEARCH ONLY
• Storage: Lyophilized samples are stable for greater than six months from date of receipt at -20 centigrade to -70 centigrade. The reconstituted samples should be stored under sterile condition in working aliquots at -20 centigrade to -70 centigrade. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended to reconstitute the lyophilized rHuFlt3-L in sterile ddH2O not less than 100μg/ml of the cytokine solution.

Gene Information

• Gene Name: FLT3LG fms-related tyrosine kinase 3 ligand [ Homo sapiens ]
• Official Symbol: FLT3LG
• Synonyms: FL; FLT3L; fms-related tyrosine kinase 3 ligand; flt3 ligand
• Gene ID: 2323
• mRNA Refseq: NM_001204502
• Protein Refseq: NP_001191431
• MIM: 600007
• UniProt ID: P49771
• Chromosome Location: 19q13.3
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem; Pathways in cancer, organism-specific biosystem
• Function: cytokine activity; protein homodimerization activity; receptor binding