Recombinant Human CD274(Phe 19 - Arg 238) fused with His tag at C-terminal was expressed in HEK293.
Predicted N Terminal:
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Has a calculated MW of 26 kDa. The reducing (R) protein migrates as 35-42 kDa in SDS-PAGE due to glycosylation.
Phe 19 - Arg 238
Less than 1.0 EU per μg of the Human PD-L1, His Tag (HPLC-verified) by the LAL method.
>98% as determined by SDS-PAGE.>95% as determined by SEC-HPLC.
Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).