Active Recombinant Human CDH2, Fc tagged

• Cat.No.: CDH2-316H
• Product Overview: Recombinant Human CDH2 fussed with Fc tag was expressed in NS0 cell.

Specification

• Description: This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.
• Source: Mammalian cells
• Species: Human
• Tag: Fc
• Predicted N Terminal: Asp160
• Form: Lyophilized from a 0.2 µm filtered solution in Tris-Citrate. See Certificate of Analysis for details.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of U-118-MG human glioblastoma/astrocytoma cells.When 5 x 10^4 cells/well are added to Recombinant Human N-Cadherin Fc Chimera coated plates (10 µg/mL, 100 µL/well), =55% will adhere after 1 hour at room temperature.
• Molecular Mass: Predicted Molecular Mass:89.2 kDa (monomer);SDS-PAGE:115 - 130 kDa, reducing conditions
• Endotoxin: < 1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Stability: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Shipping: Reconstitute at 100 µg/mL in sterile, deionized water containing 2 mM calcium 24 hours prior to use.

Gene Information

• Gene Name: CDH2 cadherin 2, type 1, N-cadherin (neuronal) [ Homo sapiens ]
• Official Symbol: CDH2
• Synonyms: CDH2; cadherin 2, type 1, N-cadherin (neuronal); NCAD; cadherin-2; CD325; CDHN; N cadherin; N-cadherin 1; neural cadherin; neural-cadherin; cadherin 2, N-cadherin (neuronal); calcium-dependent adhesion protein, neuronal; CDw325
• Gene ID: 1000
• mRNA Refseq: NM_001792
• Protein Refseq: NP_001783
• MIM: 114020
• UniProt ID: P19022
• Chromosome Location: 18q12.1
• Pathway: Adherens junctions interactions, organism-specific biosystem; Arrhythmogenic right ventricular cardiomyopathy (ARVC), organism-specific biosystem; Arrhythmogenic right ventricular cardiomyopathy (ARVC), conserved biosystem; CDO in myogenesis, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Cell junction organization, organism-specific biosystem
• Function: RPTP-like protein binding; alpha-catenin binding; beta-catenin binding; calcium ion binding; gamma-catenin binding; protein kinase binding; protein phosphatase binding