Active Recombinant Human PRSS1 Protein, His-tagged

• Cat.No.: PRSS1-516H
• Product Overview: Recombinant Human PRSS1 (Accession # NP_002760) Ala16-Ser247, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Ala16
• Form: Supplied as a 0.2 µm filtered solution in HCl and NaCl.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2.The specific activity is >3,000 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human PRSS1, His-tagged has a calculated MW of 26 kDa. In SDS-PAGE migrates as 32 kDa and 36 kDa, reducing conditions.
• Protein length: Ala16-Ser247
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: PRSS1 protease, serine, 1 (trypsin 1) [ Homo sapiens ]
• Official Symbol: PRSS1
• Synonyms: PRSS1; protease, serine, 1 (trypsin 1); TRY1; trypsin-1; beta-trypsin; trypsinogen 1; trypsinogen A; digestive zymogen; cationic trypsinogen; nonfunctional trypsin 1; TRP1; TRY4; TRYP1; FLJ57296; MGC120175; MGC149362; DKFZp779A0837
• Gene ID: 5644
• mRNA Refseq: NM_002769
• Protein Refseq: NP_002760
• MIM: 276000
• UniProt ID: P07477
• Chromosome Location: 7q34