Active Recombinant Human CDH1 Protein, Fc Chimera

• Cat.No.: CDH1-548H
• Product Overview: Recombinant Human CDH1 (Accession # NP_004351), fused with , was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: Fc
• Predicted N Terminal: Asp155
• Form: Lyophilized from a 0.2 µm filtered solution in Tris-Citrate, NaCl and CaCl2.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of the MCF-7 human breast cancer cells.When 5 x 10^4 cells/well are added to Recombinant Human E-Cadherin Fc Chimera coated plates (1.5 µg/mL with 100 µL/well), >60% cells will adhere after 90 minutes at 37 °C.Optimal concentration depends on cell type as well as the application or research objectives.
• Molecular Mass: Recombinant Human CDH1, Fc Chimera has a calculated MW of 87.7 kDa (monomer). In SDS-PAGE migrates as 120 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: CDH1 cadherin 1, type 1, E-cadherin (epithelial) [ Homo sapiens ]
• Official Symbol: CDH1
• Synonyms: CDH1; cadherin 1, type 1, E-cadherin (epithelial); UVO; cadherin-1; CD324; E Cadherin; uvomorulin; CAM 120/80; E-Cadherin; cell-CAM 120/80; epithelial cadherin; cadherin 1, E-cadherin (epithelial); calcium-dependent adhesion protein, epithelial; CDHE; ECAD; LCAM; Arc-1
• Gene ID: 999
• mRNA Refseq: NM_004360
• Protein Refseq: NP_004351
• MIM: 192090
• UniProt ID: P12830
• Chromosome Location: 16q22.1