Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-097-1828   Email: info@creative-biomart.com
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Recombinant Mouse Fgf7

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Cat.No.:
Fgf7-597M
Product Overview:
Recombinant Mouse Fgf7 (Accession # NP_032034) Cys32-Thr194, fused with , was produced in E. coli.
Source:
E. coli
Species:
Mouse
Predicted N Terminal:
Cys32
Form:
Lyophilized from a 0.2 µm filtered solution in MOPS, Na2SO4, EDTA and DTT with BSA as a carrier protein.
Bio-activity:
Measured in a cell proliferation assay using 4MBr-5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802.The ED50 for this effect is typically 10 - 50 ng/mL.
Molecular Mass:
Recombinant Mouse Fgf7 has a calculated MW of 18.7 kDa. In SDS-PAGE migrates as 19 kDa, reducing conditions.
Protein length:
Cys32-Thr194
Purity:
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage:
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitution:
Reconstitute at 10 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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