Active Recombinant Mouse Cd83 Protein, Fc Chimera

• Cat.No.: Cd83-625M
• Product Overview: Recombinant Mouse Cd83 (Accession # NP_033986), fused with , was produced in Spodoptera frugiperda, Sf 21 (stably transfected)-derived.

Specification

• Source: Sf21 Insect Cells
• Species: Mouse
• Tag: Fc
• Predicted N Terminal: Met22
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of human monocyte-derived dendritic cells.When 5 x 10^4 cells/well are added to mouse CD83/Fc coated plates (2.5 µg/mL with 100 µL/well), approximately 50 - 75% will adhere after 1 hour incubation at room temperature. Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Mouse Cd83, Fc Chimera has a calculated MW of 39.1 kDa (monomer). In SDS-PAGE migrates as 52 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Cd83 CD83 antigen [ Mus musculus ]
• Official Symbol: Cd83
• Synonyms: CD83; CD83 antigen; mCD83; MGC130312; MGC130313
• Gene ID: 12522
• mRNA Refseq: NM_009856
• Protein Refseq: NP_033986