Active Recombinant Mouse Cfh Protein, His-tagged

• Cat.No.: Cfh-626M
• Product Overview: Recombinant Mouse Cfh (Accession # NP_034018) Ser875-Val1252, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Ser875
• Form: Lyophilized from a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by the ability of the immobilized protein to induce the adhesion of human neutrophils. DiScipio, R.G. et al. (1998) J. Immunol. 160:4057.The ED50 for this effect is typically 3 - 18 µg/mL in the presence of 50 ng/mL of rhTNF-alpha.
• Molecular Mass: Recombinant Mouse Cfh, His-tagged has a calculated MW of 43.2 kDa. In SDS-PAGE migrates as 64 kDa, reducing conditions.
• Protein length: Ser875-Val1252
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 10 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: Cfh complement component factor h [ Mus musculus ]
• Official Symbol: Cfh
• Synonyms: CFH; complement component factor h; complement factor H; protein beta-1-H; complement factor H related protein 3A4/5G4; NOM; Sas1; Mud-1; Sas-1
• Gene ID: 12628
• mRNA Refseq: NM_009888
• Protein Refseq: NP_034018