Recombinant Mouse Cd47(Gln19-Pro158) fused with Fc of Human IgG1 at C-terminal was expressed in NS0.
Predicted N Terminal:
Gln19 predicted, no results obtained, sequencing might be blocked
Lyophilized from a 0.2 μm filtered solution in PBS.
Measured by its binding ability in a functional ELISA. When Recombinant Mouse SIRP alpha /CD172a Fc Chimera is coated at 2 μg/mL, Recombinant Mouse CD47 Fc Chimera binds with an apparent KD <0.6 nM.Also measured by its ability to antagonize mouse red blood cell adhesion to immobilized Recombinant Mouse SIRP alpha /CD172a Fc Chimera. The ED50 for this effect is typically 2.0-8.0 μg/mL.Optimal dilutions should be determined by each laboratory for each application.
Predicted Molecular Mass: 42.3 (monomer) kDa;SDS-PAGE: 60-70 kDa, reducing conditions
<0.01 EU per 1 μg of the protein by the LAL method.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitute at 200 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.