Active Recombinant Mouse Ahsg Protein, His-tagged

• Cat.No.: Ahsg-674M
• Product Overview: Recombinant Mouse Ahsg (Accession # NP_038493) Ala19-Ile345, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Ala19
• Form: Lyophilized from a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2.The IC50 value is <0.20 µM, as measured under the the described conditions.
• Molecular Mass: Recombinant Mouse Ahsg, His-tagged has a calculated MW of 37 kDa. In SDS-PAGE migrates as 58 kDa, reducing conditions.
• Protein length: Ala19-Ile345
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: Ahsg alpha-2-HS-glycoprotein [ Mus musculus ]
• Official Symbol: Ahsg
• Synonyms: AHSG; alpha-2-HS-glycoprotein; countertrypin; fetuin-A
• Gene ID: 11625
• mRNA Refseq: NM_013465
• Protein Refseq: NP_038493