Active Recombinant Human NRG1 Protein, His-tagged

• Cat.No.: NRG1-679H
• Product Overview: Recombinant Human NRG1 (Accession # NP_039253) Met1-Glu296, Leu117-Glu296 & Ala162-Glu296, fused with a C-terminal 6-His tag, was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.

Specification

• Source: Baculovirus Sf21 Insect Cells
• Species: Human
• Tag: His
• Predicted N Terminal: Met1, Leu117 & Ala162
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083.The ED50 for this effect is typically 1 - 5 ng/mL.
• Molecular Mass: Recombinant Human NRG1, His-tagged has a calculated MW of 33 kDa. In SDS-PAGE migrates as 25-45 kDa, reducing conditions.
• Protein length: Met1-Glu296, Leu117-Glu296 & Ala162-Glu296
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: NRG1 neuregulin 1 [ Homo sapiens ]
• Official Symbol: NRG1
• Synonyms: NRG1; neuregulin 1; HGL; pro-neuregulin-1, membrane-bound isoform; GGF; HRG; NDF; MSTP131; pro-NRG1; glial growth factor; neu differentiation factor; neuregulin 1 type IV beta 3; neuregulin 1 type IV beta 1a; sensory and motor neuron derived factor; heregulin, alpha (45kD, ERBB2 p185-activator); ARIA; GGF2; HRG1; HRGA; SMDF; MST131
• Gene ID: 3084
• mRNA Refseq: NM_001159995
• Protein Refseq: NP_001153467
• MIM: 142445
• UniProt ID: Q02297
• Chromosome Location: 8p21-p12