Active Recombinant E. coli map Protein, His-tagged

• Cat.No.: map-793E
• Product Overview: Recombinant E. coli map (Accession # NP_414710) Ala2-Glu264, fused with a C-terminal 10-His tag, was produced in E. coli.

Specification

• Source: E. coli
• Species: E. coli
• Tag: His
• Predicted N Terminal: Ala2
• Form: Supplied as a 0.2 µm filtered solution in Tris, NaCl and Glycerol.
• Bio-activity: Measure by its ability to remove methionine from a fluorogenic peptide substrate H-Met-Gly-Pro-AMC. The resulting GP-AMC is cleaved by Recombinant Human DPPIV/CD26. The specific activity is >0.5 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant E. coli map, His-tagged has a calculated MW of 31 kDa. In SDS-PAGE migrates as 36 kDa, reducing conditions.
• Protein length: Ala2-Glu264
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).