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Active Recombinant Mouse Rspo1

Cat.No. : Rspo1-816M
Product Overview : Recombinant Mouse Rspo1 (Accession # NP_619624) Met1-Gln265, fused with , was produced in Chinese Hamster Ovary cell line, CHO-derived.
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  • Gene Information
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Source : CHO Cells
Species : Mouse
Predicted N Terminal : Ser21
Form : Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Bio-activity : Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.The typical ED50 is 8-40 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a.
Molecular Mass : Recombinant Mouse Rspo1 has a calculated MW of 27 kDa. In SDS-PAGE migrates as 40-43 kDa, reducing conditions.
Protein length : Met1-Gln265
Purity : >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : Rspo1 R-spondin homolog (Xenopus laevis) [ Mus musculus ]
Official Symbol : Rspo1
Synonyms : RSPO1; R-spondin homolog (Xenopus laevis); R-spondin-1; cristin-3; mCristin-3; roof plate-specific spondin-1; thrombospondin type 1 domain containing gene Rspondin; cysteine-rich and single thrombospondin domain-containing protein 3; Rspondin; R-spondin;
Gene ID : 192199
mRNA Refseq : NM_138683
Protein Refseq : NP_619624
MIM :

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What distinguishes RSPO1 from other RSPO molecules, particularly in terms of its potential therapeutic use in Regenerative Medicine? 05/31/2022

RSPO1 stands out for its potential therapeutic use in Regenerative Medicine due to its mitogenic activity in stem cells.

What methods were employed to characterize the purified rhRSPO1, both structurally and biologically? 02/11/2022

Characterization methods included Western blotting, mass spectrometry, and in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays, confirming structural integrity and biological efficacy.

What tissue-specific processes are regulated by RSPOs, and what are some examples of these processes? 08/25/2021

RSPOs regulate various tissue-specific processes, including bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells, intestinal stem cells, and cancer progression.

Describe the purification strategy used to obtain rhRSPO1, including the chromatographic steps involved. 07/08/2021

The purification strategy for rhRSPO1 involved two sequential chromatographic steps: heparin affinity chromatography followed by molecular exclusion chromatography.

How does RSPO1 induce the Wnt pathway, and what are the downstream effects on cell proliferation, differentiation, and death? 05/19/2021

RSPO1 induces the Wnt pathway by stabilizing the Wnt receptor complex, leading to enhanced Wnt signaling and influencing cellular processes.

How was recombinant human RSPO1 (rhRSPO1) generated, and which cell line was used for this purpose? 04/08/2018

rhRSPO1 was generated using the HEK293 cell line, and the coding sequence was synthesized and subcloned into a mammalian cell expression vector.

What is the main function of R-Spondin proteins, including RSPO1, in cellular processes? 04/14/2017

R-Spondin proteins, including RSPO1, play crucial roles in cell proliferation, differentiation, and death by inducing the Wnt pathway.

Customer Reviews (3)

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Reviews
03/13/2022

    Remarkable changes in cell behavior were attributed to the potent effects of the product.

    01/16/2018

      The product's respected reputation in the scientific community was a testament to its quality and effectiveness.

      09/25/2016

        Dependable protein-protein interaction outcomes were achieved due to the product's pure composition.

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