Active Recombinant Mouse Rspo1
Cat.No. : | Rspo1-816M |
Product Overview : | Recombinant Mouse Rspo1 (Accession # NP_619624) Met1-Gln265, fused with , was produced in Chinese Hamster Ovary cell line, CHO-derived. |
- Specification
- Gene Information
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Source : | CHO Cells |
Species : | Mouse |
Predicted N Terminal : | Ser21 |
Form : | Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein. |
Bio-activity : | Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.The typical ED50 is 8-40 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a. |
Molecular Mass : | Recombinant Mouse Rspo1 has a calculated MW of 27 kDa. In SDS-PAGE migrates as 40-43 kDa, reducing conditions. |
Protein length : | Met1-Gln265 |
Purity : | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
Storage : | Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC). |
Gene Name : | Rspo1 R-spondin homolog (Xenopus laevis) [ Mus musculus ] |
Official Symbol : | Rspo1 |
Synonyms : | RSPO1; R-spondin homolog (Xenopus laevis); R-spondin-1; cristin-3; mCristin-3; roof plate-specific spondin-1; thrombospondin type 1 domain containing gene Rspondin; cysteine-rich and single thrombospondin domain-containing protein 3; Rspondin; R-spondin; |
Gene ID : | 192199 |
mRNA Refseq : | NM_138683 |
Protein Refseq : | NP_619624 |
MIM : |
Products Types
◆ Recombinant Protein | ||
RSPO1-422H | Recombinant Human RSPO1 Protein, His-tagged | +Inquiry |
RSPO1-278H | Recombinant Active Human RSPO1 Protein, His-tagged(C-ter) | +Inquiry |
RSPO1-28H | Active Recombinant Human RSPO1 Protein, Pre-aliquoted | +Inquiry |
RSPO1-132H | Active Recombinant Human RSPO1 Protein (Ser21-Ala263), N-His-SUMO tagged, Animal-free, Carrier-free | +Inquiry |
RSPO1-34H | Recombinant Human RSPO1 Protein | +Inquiry |
◆ Lysates | ||
RSPO1-001MCL | Recombinant Mouse RSPO1 cell lysate | +Inquiry |
RSPO1-001HCL | Recombinant Human RSPO1 cell lysate | +Inquiry |
RSPO1-1918HCL | Recombinant Human RSPO1 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionRSPO1 stands out for its potential therapeutic use in Regenerative Medicine due to its mitogenic activity in stem cells.
Characterization methods included Western blotting, mass spectrometry, and in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays, confirming structural integrity and biological efficacy.
RSPOs regulate various tissue-specific processes, including bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells, intestinal stem cells, and cancer progression.
The purification strategy for rhRSPO1 involved two sequential chromatographic steps: heparin affinity chromatography followed by molecular exclusion chromatography.
RSPO1 induces the Wnt pathway by stabilizing the Wnt receptor complex, leading to enhanced Wnt signaling and influencing cellular processes.
rhRSPO1 was generated using the HEK293 cell line, and the coding sequence was synthesized and subcloned into a mammalian cell expression vector.
R-Spondin proteins, including RSPO1, play crucial roles in cell proliferation, differentiation, and death by inducing the Wnt pathway.
Customer Reviews (3)
Write a reviewRemarkable changes in cell behavior were attributed to the potent effects of the product.
The product's respected reputation in the scientific community was a testament to its quality and effectiveness.
Dependable protein-protein interaction outcomes were achieved due to the product's pure composition.
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