Recombinant Human ATRN(Ala84-Gln1272) fused with His tag at C-terminal was expressed in CHO.
Predicted N Terminal:
Lyophilized from a 0.2 μm filtered solution in PBS.
Measured by its binding ability in a functional ELISA. When recombinant mouse Agouti is coated at 1 μg/mL, Recombinant Human Attractin binds with apparent KD <40 nM.
Predicted Molecular Mass: 135 kDa;SDS-PAGE: 150-180 kDa, reducing conditions
<0.10 EU per 1 μg of the protein by the LAL method.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitute at 500 μg/mL in PBS.