Active Recombinant Human ANGPT2 Protein, His-tagged

• Cat.No.: ANGPT2-933H
• Product Overview: Recombinant Human ANGPT2 (Accession # O15123) Tyr19-Phe496, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Tyr19
• Form: Lyophilized from a 0.2 µm filtered solution in MOPS, NaCl, CHAPS and PEG with BSA as a carrier protein.
• Bio-activity: Measured by its ability to activate Tie-2 in C6 rat glial cells transfected with human Tie-2.0.2 µg/mL of Recombinant Human Angiopoietin-2 significantly induces phosphorylation of human Tie-2.
• Molecular Mass: Recombinant Human ANGPT2, His-tagged has a calculated MW of 50.1 kDa. In SDS-PAGE migrates as 66 kDa, reducing conditions.
• Protein length: Tyr19-Phe496
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: ANGPT2 angiopoietin 2 [ Homo sapiens ]
• Official Symbol: ANGPT2
• Synonyms: ANGPT2; angiopoietin 2; angiopoietin-2; Ang2; ANG-2; Tie2-ligand; angiopoietin-2B; angiopoietin-2a; ANG2; AGPT2
• Gene ID: 285
• mRNA Refseq: NM_001118887
• Protein Refseq: NP_001112359
• MIM: 601922
• UniProt ID: O15123
• Chromosome Location: 8p23