Recombinant Human OGT(Cys323-Glu1041) fused with His tag at C-terminal was expressed in E. coli.
Predicted N Terminal:
Supplied as a 0.2 μm filtered solution in Tris, NaCl and TCEP.
Measured by its ability to transfer GlcNAc from UDP-GclNAc to peptide OGT substrate. The specific activity is >25 pmol/min/μg.
Predicted Molecular Mass: 81 kDa;SDS-PAGE: 70 kDa, reducing conditions
<1.0 EU per 1 μg of the protein by the LAL method.
>80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
Avoid repeated freeze-thaw cycles.6 months from date of receipt, -20 to -70 centigrade as supplied.3 months, -20 to -70 centigrade under sterile conditions after opening.