Active Recombinant Porcine IL18 Protein, His-tagged
Cat.No. : | IL18-946P |
Product Overview : | Recombinant Porcine IL18 (Accession # O19073) Tyr36-Asn192, fused with a C-terminal 6-His tag, was produced in E. coli. |
- Specification
- Gene Information
- Related Products
Source : | E. coli |
Species : | Porcine |
Tag : | His |
Predicted N Terminal : | Tyr36 |
Form : | Lyophilized from a 0.2 µm filtered solution in PBS and DTT with Trehalose and with BSA as a carrier protein. |
Bio-activity : | Measured by its ability to induce IFN-gamma secretion by KG-1 human acute myelogenous leukemia cells in the presence of TNF-alpha.The ED50 for this effect is typically 10 - 40 ng/mL in the presence of 10 ng/mL rhTNF-alpha. |
Molecular Mass : | Recombinant Porcine IL18, His-tagged has a calculated MW of 18.0 kDa. |
Protein length : | Tyr36-Asn192 |
Purity : | >97%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
Storage : | Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC). |
Gene Name : | CHST10 carbohydrate sulfotransferase 10 [ Sus scrofa (pig) ] |
Official Symbol : | CHST10 |
Synonyms : | CHST10; carbohydrate sulfotransferase 10 |
Gene ID : | 1.0052e+008 |
mRNA Refseq : | XM_003124889.4 |
Protein Refseq : | XP_003124937.3 |
MIM : | |
Chromosome Location : | chromosome: 3 |
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionCo-immunoprecipitation, co-localization studies, and pathway inhibition assays decipher IL18's interactions with signaling molecules and pathways.
Therapeutic antibody trials, in vitro drug screens, and disease model assessments assess IL18-targeted interventions in autoimmune diseases.
Xenograft models, intravital imaging, and tissue-specific knockout approaches unravel IL18's kinetics and effects within complex in vivo environments.
Flow cytometry, chemotaxis assays, and functional cell assays examine IL18's impact on immune cell activation, migration, and effector functions.
Surface plasmon resonance, mutational studies, and phosphoproteomics illuminate IL18's receptor interactions and downstream signal transduction pathways.
Immunophenotyping, cytokine profiling, and gene expression analyses elucidate IL18's influence on Th cell polarization and immune balance.
ChIP assays, promoter analyses, and epigenetic profiling uncover the regulatory mechanisms underlying IL18 gene expression dynamics.
Disease models, knockout mice, and co-stimulation studies explore IL18's involvement in inflammatory conditions and its interplay with other proinflammatory mediators.
Tumor models, immune profiling, and immunotherapeutic studies investigate IL18's modulation of the tumor microenvironment and its implications for cancer therapy.
Caspase assays, cytokine ELISAs, and microscopy techniques unveil the activation of inflammasomes and IL18's contribution to pyroptosis and cytokine storms.
Customer Reviews (3)
Write a reviewEffective in inflammation models.
High stability in serum.
Suitable for ELISA.
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