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Active Recombinant Porcine IL18 Protein, His-tagged

Cat.No. : IL18-946P
Product Overview : Recombinant Porcine IL18 (Accession # O19073) Tyr36-Asn192, fused with a C-terminal 6-His tag, was produced in E. coli.
  • Specification
  • Gene Information
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Source : E. coli
Species : Porcine
Tag : His
Predicted N Terminal : Tyr36
Form : Lyophilized from a 0.2 µm filtered solution in PBS and DTT with Trehalose and with BSA as a carrier protein.
Bio-activity : Measured by its ability to induce IFN-gamma secretion by KG-1 human acute myelogenous leukemia cells in the presence of TNF-alpha.The ED50 for this effect is typically 10 - 40 ng/mL in the presence of 10 ng/mL rhTNF-alpha.
Molecular Mass : Recombinant Porcine IL18, His-tagged has a calculated MW of 18.0 kDa.
Protein length : Tyr36-Asn192
Purity : >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Gene Name : CHST10 carbohydrate sulfotransferase 10 [ Sus scrofa (pig) ]
Official Symbol : CHST10
Synonyms : CHST10; carbohydrate sulfotransferase 10
Gene ID : 1.0052e+008
mRNA Refseq : XM_003124889.4
Protein Refseq : XP_003124937.3  
MIM :
Chromosome Location : chromosome: 3

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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How do researchers experimentally investigate the crosstalk between IL18 and other immune signaling pathways, such as Toll-like receptor signaling? 10/28/2022

Co-immunoprecipitation, co-localization studies, and pathway inhibition assays decipher IL18's interactions with signaling molecules and pathways.

What assays and methodologies are employed to evaluate the potential therapeutic interventions targeting IL18 in autoimmune diseases and chronic inflammation? 03/23/2022

Therapeutic antibody trials, in vitro drug screens, and disease model assessments assess IL18-targeted interventions in autoimmune diseases.

How are animal models and in vivo imaging techniques used to understand the kinetics of IL18-mediated immune responses and its tissue-specific effects? 01/10/2022

Xenograft models, intravital imaging, and tissue-specific knockout approaches unravel IL18's kinetics and effects within complex in vivo environments.

Can you elaborate on the techniques used to assess the impact of IL18 on immune cell activation, migration, and effector functions? 06/11/2021

Flow cytometry, chemotaxis assays, and functional cell assays examine IL18's impact on immune cell activation, migration, and effector functions.

Can you explain the experimental strategies used to analyze the interactions between IL18 and its receptor complex, as well as the downstream signaling events triggered by receptor engagement? 01/12/2021

Surface plasmon resonance, mutational studies, and phosphoproteomics illuminate IL18's receptor interactions and downstream signal transduction pathways.

How are cellular and molecular techniques employed to investigate IL18's role in regulating the balance between Th1 and Th2 immune responses? 08/01/2020

Immunophenotyping, cytokine profiling, and gene expression analyses elucidate IL18's influence on Th cell polarization and immune balance.

What experimental approaches are utilized to study the genetic and epigenetic regulation of IL18 expression in response to different stimuli? 04/29/2020

ChIP assays, promoter analyses, and epigenetic profiling uncover the regulatory mechanisms underlying IL18 gene expression dynamics.

What methods are used to dissect the mechanisms through which IL18 contributes to the pathogenesis of inflammatory diseases and its interactions with other proinflammatory molecules? 12/15/2018

Disease models, knockout mice, and co-stimulation studies explore IL18's involvement in inflammatory conditions and its interplay with other proinflammatory mediators.

How do researchers investigate the role of IL18 in modulating the tumor microenvironment and its potential implications for cancer immunotherapy? 11/05/2018

Tumor models, immune profiling, and immunotherapeutic studies investigate IL18's modulation of the tumor microenvironment and its implications for cancer therapy.

Could you explain the experimental strategies used to characterize the inflammasome activation triggered by IL18 and its downstream effects on cytokine production? 04/20/2018

Caspase assays, cytokine ELISAs, and microscopy techniques unveil the activation of inflammasomes and IL18's contribution to pyroptosis and cytokine storms.

Customer Reviews (3)

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Reviews
10/15/2022

    Effective in inflammation models.

    08/22/2021

      High stability in serum.

      05/09/2019

        Suitable for ELISA.

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