Active Recombinant Human CA12 Protein, His-tagged
Cat.No. : | CA12-971H |
Product Overview : | Recombinant Human CA12 (Accession # O43570) Ala25-Gln291, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived. |
- Specification
- Gene Information
- Related Products
Source : | Mammalian cells |
Species : | Human |
Tag : | His |
Predicted N Terminal : | Ala25 |
Form : | Supplied as a 0.2 µm filtered solution in Tris and NaCl. |
Bio-activity : | Measured by its esterase activity. The specific activity is >10 pmol/min/µg, as measured under the described conditions. |
Molecular Mass : | Recombinant Human CA12, His-tagged has a calculated MW of 32 kDa. In SDS-PAGE migrates as 38-41 kDa, reducing conditions. |
Protein length : | Ala25-Gln291 |
Purity : | >95%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
Storage : | Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC). |
Reconstitution : | Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
Gene Name : | CA12 carbonic anhydrase XII [ Homo sapiens ] |
Official Symbol : | CA12 |
Synonyms : | CA12; carbonic anhydrase XII; carbonic anhydrase 12; HsT18816; CA-XII; carbonic dehydratase; carbonate dehydratase XII; tumor antigen HOM-RCC-3.1.3; CAXII; FLJ20151; |
Gene ID : | 771 |
mRNA Refseq : | NM_001218 |
Protein Refseq : | NP_001209 |
MIM : | 603263 |
UniProt ID : | O43570 |
Chromosome Location : | 15q22 |
Products Types
◆ Recombinant Protein | ||
CA12-0233H | Recombinant Human CA12 Protein, GST-Tagged | +Inquiry |
Car12-1958M | Recombinant Mouse Car12 Protein, Myc/DDK-tagged | +Inquiry |
CA12-260M | Active Recombinant Mouse CA12 protein(Met1-Ser301), His-tagged | +Inquiry |
CA12-2032H | Recombinant Human CA12 Protein, MYC/DDK-tagged | +Inquiry |
CA12-1154M | Recombinant Mouse CA12 Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
CA12-3067MCL | Recombinant Mouse CA12 cell lysate | +Inquiry |
CA12-3061HCL | Recombinant Human CA12 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (7)
Ask a questionKnown inhibitors can be identified through drug screening assays, and their impact on CA12 can be studied using kinetic studies and cell-based assays.
Cellular localization can be identified using immunofluorescence, subcellular fractionation, or electron microscopy.
CA12 has been implicated in tumor progression, particularly in hypoxic environments, by modulating pH and thus influencing tumor cell survival and metastasis. This can be assessed using cellular assays and tumor models.
Downstream pathways can be identified using pathway analysis tools, phosphoproteomics, and specific signal transduction assays.
The activity modulation under different pH conditions can be determined using enzymatic assays and pH shift experiments.
Expression changes in response to stressors can be analyzed using techniques like qPCR, Western blotting, or proteomics following cellular treatment with specific agents.
Genetic variants and their impact can be determined using structural models and functional assays comparing wild-type and mutant proteins.
Customer Reviews (3)
Write a reviewProfessional assistance, invaluable for complex protein experiments.
Cost-effective quantitation, ideal for budget-conscious labs.
Impressive expertise, accelerates our research progress.
Ask a Question for All CA12 Products
Required fields are marked with *
My Review for All CA12 Products
Required fields are marked with *
Inquiry Basket