Active Recombinant Mouse Pcdh12 Protein, His-tagged

• Cat.No.: Pcdh12-991M
• Product Overview: Recombinant Mouse Pcdh12 (Accession # O55134) Leu18-Ala716, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: No results obtained, Leu18 predicted. Protein identity confirmed by MS analysis of tryptic fragments.
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells.The ED50 for this effect is typically 0.15 - 0.9 µg/mL.
• Molecular Mass: Recombinant Mouse Pcdh12, His-tagged has a calculated MW of 77 kDa (monomer). In SDS-PAGE migrates as 85-105 kDa, reducing conditions.
• Protein length: Leu18-Ala716
• Purity: >95%, by SDS-PAGE with silver staining.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: Pcdh12 protocadherin 12 [ Mus musculus ]
• Official Symbol: Pcdh12
• Synonyms: PCDH12; protocadherin 12; protocadherin-12; VE-cadherin-2; protocadherin 14; vascular cadherin-2; vascular endothelial cadherin-2; Pcdh14; VE-cad-2
• Gene ID: 53601
• mRNA Refseq: NM_017378
• Protein Refseq: NP_059074