Active Recombinant Human TPST1 Protein, His-tagged

• Cat.No.: TPST1-1002H
• Product Overview: Recombinant Human TPST1 (Accession # O60507) Gln26-Glu370, fused with an N-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: His
• Form: Supplied as a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to transfer sulfate from PAPS to PSGL-1 peptide (Gln-Ala-Thr-Glu-Tyr-Glu-Tyr-Leu-Asp-Tyr-Asp-Phe-Leu-Pro-Glu-Thr)The specific activity is >5 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human TPST1, His-tagged has a calculated MW of 40 kDa. In SDS-PAGE migrates as 40-50 kDa, reducing conditions.
• Protein length: Gln26-Glu370
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: TPST1 tyrosylprotein sulfotransferase 1 [ Homo sapiens ]
• Official Symbol: TPST1
• Synonyms: TPST1; tyrosylprotein sulfotransferase 1; protein-tyrosine sulfotransferase 1; TPST-1; tyrosylprotein sulfotransferase-1
• Gene ID: 8460
• mRNA Refseq: NM_003596
• Protein Refseq: NP_003587
• MIM: 603125
• UniProt ID: O60507
• Chromosome Location: 7q11.21