Active Recombinant Mouse Il27ra Protein, Fc Chimera

• Cat.No.: Il27ra-1013M
• Product Overview: Recombinant Mouse Il27ra (Accession # O70394), fused with , was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: Fc
• Predicted N Terminal: Gly29
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to inhibit IL-27-induced proliferation of mouse CD4+ T cells.The ED50 for this effect is typically 1-4 µg/mL in the presence of 10 ng/mL of recombinant mouse IL-27.Measured by its ability to inhibit IL-27 anti-viral activity using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Bender H. et al. (2009) Hepatology 50:585.The ED50 for this effect is typically 2-12 µg/mL.
• Molecular Mass: Recombinant Mouse Il27ra, Fc Chimera has a calculated MW of 80 kDa (monomer). In SDS-PAGE migrates as 105-115 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: Il27ra interleukin 27 receptor, alpha [ Mus musculus ]
• Official Symbol: Il27ra
• Synonyms: IL27RA; interleukin 27 receptor, alpha; interleukin-27 receptor subunit alpha; IL-27RA; IL-27R-alpha; IL-27R subunit alpha; T cell cytokine receptor; IL-27 receptor subunit alpha; type I T-cell cytokine receptor; cytokine receptor family, class 1 (WSXWS), member 1; CRL1; Tccr; Wsx1; WSX-1; IL-27R; zcytor1
• Gene ID: 50931
• mRNA Refseq: NM_016671
• Protein Refseq: NP_057880