Active Recombinant Zebrafish vegfaa

• Cat.No.: vegfaa-1019Z
• Product Overview: Recombinant Zebrafish vegfaa (Accession # O73682.1) Ala24-Arg188, fused with , was produced in Spodoptera frugiperda, Sf 21 (stably transfected)-derived.

Specification

• Source: Sf21 Insect Cells
• Species: Zebrafish
• Predicted N Terminal: Ala24
• Form: Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Bio-activity: Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is typically 20-60 ng/mL.
• Molecular Mass: Recombinant Zebrafish vegfaa has a calculated MW of 19.2 kDa (monomer). In SDS-PAGE migrates as 22 kDa, reducing conditions.
• Protein length: Ala24-Arg188
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: vegfaa vascular endothelial growth factor Aa [ Danio rerio (zebrafish) ]
• Official Symbol: vegfaa
• Synonyms: vegf; vegfa; wu:fj82c06; vascular endothelial growth factor A-A; vascular endothelial growth factor A-A; VEGF-A-A; etID309976.16; vascular endothelial growth factor A; vegf-a
• Gene ID: 30682
• mRNA Refseq: NM_001110349.2
• Protein Refseq: NP_001103819.2
• UniProt ID: O73682
• Chromosome Location: chromosome: 16