Active Recombinant Human ENTPD3 Protein, His-tagged

• Cat.No.: ENTPD3-1033H
• Product Overview: Recombinant Human ENTPD3 (Accession # O75355) Gln44-Pro485, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: No results obtained: Gln44 predicted
• Form: Supplied as a 0.2 µm filtered solution in Tris, CaCl2, NaCl and Glycerol.
• Bio-activity: Measured by its ability to hydrolyze the 5"-phosphate groups from the substrate adenosine-5"-triphosphate (ATP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit.The specific activity is >70,000 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human ENTPD3, His-tagged has a calculated MW of 50 kDa. In SDS-PAGE migrates as 65-80 kDa, reducing conditions.
• Protein length: Gln44-Pro485
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: ENTPD3 ectonucleoside triphosphate diphosphohydrolase 3 [ Homo sapiens ]
• Official Symbol: ENTPD3
• Synonyms: ENTPD3; ectonucleoside triphosphate diphosphohydrolase 3; CD39L3; HB6; NTPDase 3; CD39-like 3; ecto-ATPase 3; ecto-ATPDase 3; ecto-apyrase 3; CD39 antigen-like 3; ecto-ATP diphosphohydrolase 3; NTPDase-3; FLJ93839
• Gene ID: 956
• mRNA Refseq: NM_001248
• Protein Refseq: NP_001239
• MIM: 603161
• UniProt ID: O75355
• Chromosome Location: 3p21.3