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Home > Products > Recombinant Proteins > Recombinant Human CRLF1&CLCF1

Recombinant Human CRLF1&CLCF1

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Product Overview:
Recombinant Human CRLF1&CLCF1 (Accession # O75462 (CLF-1) & Q9UBD9 (CLC)), fused with , was produced in Mouse myeloma cell line, NS0-derived.
NSO Cells
Predicted N Terminal:
Ala38 (CLF-1) & Leu28 (CLC)
Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells transfected with human CNTF R alpha.The ED50 for this effect is typically 0.5 - 3.5 ng/mL.
Molecular Mass:
Recombinant Human CRLF1&CLCF1 has a calculated MW of 44.5 kDa (CLF-1) & 23 kDa (CLC). In SDS-PAGE migrates as 50-65 kDa (CLF-1) & 28-30 kDa (CLC), reducing conditions.
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
Reconstitute at 200 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127

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