Active Recombinant Human CST7 Protein, His-tagged

• Cat.No.: CST7-1047H
• Product Overview: Recombinant Human CST7 (Accession # O76096) Gly20-His145, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Gly20
• Form: Lyophilized from a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC.The IC50 value is <6.0 nM, as measured under the described conditions.
• Molecular Mass: Recombinant Human CST7, His-tagged has a calculated MW of 15 kDa. In SDS-PAGE migrates as 16 kDa, 21 kDa and 26 kDa, reducing conditions.
• Protein length: Gly20-His145
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: CST7 cystatin F (leukocystatin) [ Homo sapiens ]
• Official Symbol: CST7
• Synonyms: CST7; cystatin F (leukocystatin); cystatin-F; cystatin-7; leukocystatin; cystatin-like metastasis-associated protein; CMAP
• Gene ID: 8530
• mRNA Refseq: NM_003650
• Protein Refseq: NP_003641
• MIM: 603253
• UniProt ID: O76096
• Chromosome Location: 20p11.21