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Active Recombinant Human ALDH1A1 protein, His-tagged

Cat.No. : ALDH1A1-1100H
Product Overview : Recombinant Human ALDH1A1(Ser2-Ser501) fused with an N-terminal Met and 6-His tag was expressed in E. coli.
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  • Gene Information
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Description : Aldehyde dehydrogenases (ALDHs) are NAD(P)+-dependent enzymes that detoxify aldehydes by oxidizing them to carboxylic acids. Nineteen ALDHs are present in humans, expressed in a variety of organelles and having different substrate preferences. ALDH1A1 is a cytosolic enzyme that preferentially oxidizes retinaldehyde to retinoic acid. ALDH1A1 is expressed in the epithelium of many organs, including brain, liver, testis, eye lens and cornea. ALDH1A1 is highly expressed in brain dopaminergic neurons, where it produces the retinoic acid required for their differentiation and development. The retinoic acid produced by ALDH1A1 is also important for the differentiation of hematopoietic stem cells. ALDH1A1 is a major enzyme in the oxidation of acetaldehyde, a toxic metabolite of ethanol.
Source : E. coli
Species : Human
Tag : His
Predicted N Terminal : Ser2
Form : Supplied as a 0.2 μm filtered solution in Tris, NaCl, Glycerol and DTT.
Bio-activity : Measured by its ability to produce NADH during the oxidation of propionaldehyde. The specific activity is >150 pmol/min/μg.
Molecular Mass : Predicted Molecular Mass: 56 kDa
SDS-PAGE: 57 kDa, reducing conditions
Endotoxin : <1.0 EU per 1 μg of the protein by the LAL method.
Purity : >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage : Avoid repeated freeze-thaw cycles.
6 months from date of receipt, -70 centigrade as supplied.
3 months, -70 centigrade under sterile conditions after opening.
Gene Name : ALDH1A1 aldehyde dehydrogenase 1 family, member A1 [ Homo sapiens ]
Official Symbol : ALDH1A1
Synonyms : ALDH1A1; aldehyde dehydrogenase 1 family, member A1; ALDH1, PUMB1; retinal dehydrogenase 1; RALDH1; retinaldehyde dehydrogenase 1; ALHDII; RALDH 1; ALDH class 1; acetaldehyde dehydrogenase 1; aldehyde dehydrogenase 1, soluble; aldehyde dehydrogenase, liver cytosolic; ALDC; ALDH1; PUMB1; ALDH11; ALDH-E1; MGC2318;
Gene ID : 216
mRNA Refseq : NM_000689
Protein Refseq : NP_000680
MIM : 100640
UniProt ID : P00352
Chromosome Location : 9q21.13
Pathway : Biological oxidations, organism-specific biosystem; Ethanol oxidation, organism-specific biosystem; Fatty Acid Omega Oxidation, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Phase 1 - Functionalization of compounds, organism-specific biosystem; Retinol metabolism, organism-specific biosystem;
Function : Ras GTPase activator activity; aldehyde dehydrogenase (NAD) activity; androgen binding; oxidoreductase activity; retinal dehydrogenase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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How does ALDH1A1 contribute to cellular detoxification by metabolizing endogenous and exogenous aldehydes? 09/26/2021

ALDH1A1 contributes to cellular detoxification by metabolizing endogenous and exogenous aldehydes, reducing their potential cytotoxic effects.

What are the structural features of ALDH1A1 that are crucial for its catalytic activity, and how do substrate binding and cofactor interaction occur? 08/07/2021

ALDH1A1's catalytic activity depends on its conserved structural features. Substrate binding involves the aldehyde-binding pocket, and cofactor interaction occurs through the binding of NAD+.

How does ALDH1A1 participate in retinoid homeostasis, and what are the consequences of its dysregulation in diseases like vitamin A deficiency or excess? 12/04/2020

ALDH1A1 contributes to retinoid homeostasis by regulating the levels of active retinoic acid. Dysregulation may lead to diseases associated with vitamin A deficiency or excess.

What is the enzymatic function of ALDH1A1, and how does it catalyze the conversion of aldehydes? 08/14/2020

ALDH1A1 is an aldehyde dehydrogenase enzyme responsible for catalyzing the oxidation of retinaldehyde to retinoic acid, a critical step in the synthesis of retinoids.

Can you explain the role of ALDH1A1 in retinoic acid signaling and its impact on cellular processes, including gene expression and differentiation? 05/07/2018

ALDH1A1 plays a key role in retinoic acid signaling by generating active retinoic acid. This signaling pathway influences gene expression and cellular differentiation in various tissues.

Customer Reviews (3)

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Reviews
08/05/2020

    The attention to detail in quality assurance is commendable. It's a lab favorite for a reason.

    03/12/2019

      Great for assays and experiments. Dependable results make it a lab favorite.

      11/23/2018

        A lab staple! Quality is never compromised, and the shipping is always reliable.

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