Active Recombinant Human PNP Protein, His-tagged

• Cat.No.: PNP-1101H
• Product Overview: Recombinant Human PNP (Accession # P00491) Met1-Ser289, fused with a C-terminal 6-His tag, was produced in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Predicted N Terminal: Met1
• Form: Supplied as a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by the phosphorolysis of 7-methyl-6-thioguanosine.The specific activity is >35,000 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human PNP, His-tagged has a calculated MW of 33 kDa. In SDS-PAGE migrates as 30-35 kDa, reducing conditions.
• Protein length: Met1-Ser289
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: PNP purine nucleoside phosphorylase [ Homo sapiens ]
• Official Symbol: PNP
• Synonyms: PNP; purine nucleoside phosphorylase; NP, nucleoside phosphorylase; PUNP; inosine phosphorylase; purine-nucleoside:orthophosphate ribosyltransferase; NP; PRO1837; FLJ94043; FLJ97288; FLJ97312; MGC117396; MGC125915; MGC125916
• Gene ID: 4860
• mRNA Refseq: NM_000270
• Protein Refseq: NP_000261
• MIM: 164050
• UniProt ID: P00491
• Chromosome Location: 14q11.2