Active Recombinant Human PGA4 Protein, His-tagged

• Cat.No.: PGA4-1110H
• Product Overview: Recombinant Human PGA4 (Accession # P00790) Met1-Ala388, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Ile16 and Val63
• Form: Supplied as a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2.The specific activity is >7,000 pmol/min/µg, as measured under the described conditions. See Activity Assay Protocol on www.RnDSystems.com.
• Molecular Mass: Recombinant Human PGA4, His-tagged has a calculated MW of 41 kDa. In SDS-PAGE migrates as 40-43 kDa, reducing conditions.
• Protein length: Met1-Ala388
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: PGA4 pepsinogen 4, group I (pepsinogen A) [ Homo sapiens (human) ]
• Official Symbol: PGA4
• Synonyms: pepsin A-4; pepsin A-4; pepsin A; pepsinogen A4; pepsinogen-3; pepsinogen-4
• Gene ID: 643847
• mRNA Refseq: NM_001079808.2
• Protein Refseq: NP_001073276.1
• MIM: 169720
• UniProt ID: P0DJD7
• Chromosome Location: 11q12.2