Active Recombinant Human NCR3LG1, Fc-tagged, Biotinylated

• Cat.No.: NCR3LG1-547H
• Product Overview: The recombinant human B7-H6-Fc fusion is expressed as a 466 amino acid protein consisting of Asp25 - Ser262 region of B7-H6 (UniProt accession #Q68D85) and a C-terminal Fc fusion from human IgG1, which exists as a dimer under non-reducing condition.

Specification

• Source: Human cells
• Species: Human
• Tag: Fc
• Form: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule).
• Bio-activity: Binds to its receptor NKp30/NCR3 and Induces IFN-γ secretion by NK-92 human natural killer lymphoma cells with an ED50 of 0.4 - 2.6 μg/ml.
• Molecular Mass: Calculated molecular mass 52.2 kDa; estimated by SDS-PAGE under reducing condition 75-85 kDa probably due to glycosylation
• AA Sequence: DLKVEMMAGGTQITPLNDNVTIFCN IFYSQPLNITSMGITWFWKSLTFDK EVKVFEFFGDHQEAFRPGAIVSPW RLKSGDASLRLPGIQLEEAGEYRCE VVVTPLKAQGTVQLEVVASPASRLL LDQVGMKENEDKYMCESSGFYPEA INITWEKQTQKFPHPIEISEDVITG PTIKNMDGTFNVTSCLKLNSSQEDP GTVYQCVVRHASLHTPLRSNFTLT AARHSLSETEKTDNFSSTGTHTCPP CPAPELLGGPSVFLFPPKPKDTLMI SRTPEVTCVVVDVSHEDPEVKFNW YVDGVEVHNAKTKPREEQYNSTYRV VSVLTVLHQDWLNGKEYKCKVSNKA LPAPIEKTISKAKGQPREPQVYTL PPSREEMTKNQVSLTCLVKGFYPSD IAVEWESNGQPENNYKTTPPVLDSD GSFFLYSKLTVDKSRWQQGNVFSC SVMHEALHNHYTQKSLSLSPGK
• Endotoxin: <0.1 eu per 1 μg of purified recombinant protein determined by the
• Purity: >95% judged by SDS-PAGE under reducing condition
• Storage: The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: NCR3LG1 natural killer cell cytotoxicity receptor 3 ligand 1 [ Homo sapiens ]
• Official Symbol: NCR3LG1
• Synonyms: B7H6; B7-H6; DKFZp686O24166; DKFZp686I21167; natural cytotoxicity triggering receptor 3 ligand 1; B7 homolog 6; putative Ig-like domain-containing protein DKFZp686O24166/DKFZp686I21167
• Gene ID: 374383
• mRNA Refseq: NM_001202439
• Protein Refseq: NP_001189368
• MIM: 613714
• UniProt ID: Q68D85
• Chromosome Location: 11p15.1
• Function: structural molecule activity