Active Recombinant Human CDNF, His-tagged, Biotinylated

• Cat.No.: CDNF-591H
• Product Overview: The recombinant human CDNF is expressed as a 174 amino acid protein consisting of Gln25 - Leu187 region of CDNF (UniProt entry Q49AH0) and a C-terminal poly-Histidine tag.

Specification

• Source: Human cells
• Species: Human
• Tag: His
• Form: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule).
• Bio-activity: CDNF is reported to enhance neurite outgrowth of E18 rat embryonic cortical neurons and increases neurite outgrowth when immobilized at 6 - 24 μg/mL on a nitrocellulose- coated microplate.
• Molecular Mass: Calculated molecular mass 19.9 kDa; estimated by SDS-PAGE under reducing condition ~22 kDa with a higher molecular mass band (~25 kDa) resembling a glycosylated state.
• AA Sequence: QGQEAGGRPGADCEVCKEFLNRFYK SLIDRGVNFSLDTIEKELISFCLDT KGKENRLCYYLGATKDAATKILSEV TRPMSVHMPAMKICEKLKKLDSQIC ELKYEKTLDLASVDLRKMRVAELKQ ILHSWGEECRACAEKTDYVNLIQE LAPKYAATHPKTELSTGHHHHHHHH
• Endotoxin: <0.1 eu per 1 μg of purified recombinant protein determined by the
• Purity: >95% judged by SDS-PAGE under reducing condition
• Storage: The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: CDNF cerebral dopamine neurotrophic factor [ Homo sapiens ]
• Official Symbol: CDNF
• Synonyms: CDNF; cerebral dopamine neurotrophic factor; arginine rich, mutated in early stage tumors like 1 , ARMETL1; conserved dopamine neurotrophic factor; ARMET-like protein 1; arginine-rich, mutated in early stage tumors-like 1; ARMETL1
• Gene ID: 441549
• mRNA Refseq: NM_001029954
• Protein Refseq: NP_001025125
• MIM: 611233
• UniProt ID: Q49AH0
• Chromosome Location: 10p13
• Function: growth factor activity