Nickel Beads
Cat.No. : | Nickel-31A |
Product Overview : | Recombinant proteins tagged with 6-10 poly Histidines can be purified in one step by ion metal affinity chromatography (IMAC). Several metal ions exhibiting affinity to poly-Histidines are commonly used, Nickel and Cobalt being the most popular. Tagging the proteins may be done at the N-ter or the C-ter by cloning a gene in dedicated expression vectors encoding to the poly Histidines stretch, enabling the purification of the recombinant protein resulted. |
- Specification
- Gene Information
- Related Products
Cat.no : | Nickel-31A |
Matrix : | Highly cross-linked spherical agarose, 4% |
Average particle size : | 40‐165 μm |
Dynamic binding capacity : | ~6-9 mg pure (His) 6 -tagged protein (Mr ~ 49 000) per ml |
Recommended flow rate : | 1-2 ml/min/ cm2 |
pH working range : | 2-11 |
Elution buffer : | 0.02 M sodium phosphate, 0.5 M NaCl, 0.5 M imidazole, pH 7.4 |
Storage : | Storage conditions: equilibrate the column with the Binding buffer added with NaN3 0.1% (w/v) or 20% ethanol as preservatives. |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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