Active Recombinant Mouse Rspo1, Fc-tagged, Biotinylated

• Cat.No.: Rspo1-723M
• Product Overview: The recombinant mouse RPSO1-Fc fusion is expressed as a 483 amino acid protein consisting of Ser21 - Gln265 region of (UniProt accession #Q9Z132) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

Specification

• Source: Human cells
• Species: Mouse
• Tag: Fc
• Form: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule).
• Bio-activity: Synergizes with Wnt3a to stabilize β-catenin protein levels and induces the activation of β-catenin responsive Luciferase reporter gene in HEK293 cells with a typical ED50 of 60 - 300 ng/ml.
• Molecular Mass: Calculated molecular mass (kDa): 53.8; Estimated by SDS-PAGE under reducing condition (kDa): 55-60.
• AA Sequence: SRGIKGKRQRRISAEGSQACAKGCE LCSEVNGCLKCSPKLFILLERNDIR QVGVCLPSCPPGYFDARNPDMNKCI KCKIEHCEACFSHNFCTKCQEGLYL HKGRCYPACPEGSTAANSTMECGSP AQCEMSEWSPWGPCSKKRKLCGFR KGSEERTRRVLHAPGGDHTTCSDTK ETRKCTVRRTPCPEGQKRRKGGQGR RENANRHPARKNSKEPGSNSRRHK GQQQPQPGTTGPLTSVGPTWAQSTT ENLYFQGSTGTHTCPPCPAPELLGG PSVFLFPPKPKDTLMISRTPEVTC VVVDVSHEDPEVKFNWYVDGVEVHN AKTKPREEQYNSTYRVVSVLTVLHQ DWLNGKEYKCKVSNKALPAPIEKT ISKAKGQPREPQVYTLPPSREEMTK NQVSLTCLVKGFYPSDIAVEWESNG QPENNYKTTPPVLDSDGSFFLYSK LTVDKSRWQQGNVFSCSVMHEALHN HYTQKSLSLSPGK
• Endotoxin: <0.1 eu per 1 μg of purified recombinant protein determined by the lal>
• Purity: >95% judged by SDS-PAGE under reducing condition
• Storage: The product is shipped at 4°C. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20°C for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4°C for no more than 2 weeks. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: Rspo1 R-spondin homolog (Xenopus laevis) [ Mus musculus ]
• Official Symbol: Rspo1
• Synonyms: RSPO1; R-spondin homolog (Xenopus laevis); R-spondin-1; cristin-3; mCristin-3; roof plate-specific spondin-1; thrombospondin type 1 domain containing gene Rspondin; cysteine-rich and single thrombospondin domain-containing protein 3; Rspondin; R-spondin
• Gene ID: 192199
• mRNA Refseq: NM_138683
• Protein Refseq: NP_619624
• Function: heparin binding; receptor binding