Recombinant Human Thiopurine S-methyltransferase

• Cat.No.: TPMT-76H
• Product Overview: Recombinant human TPMT was expressed in E.coli and purified by using conventional chromatography techniques(245 aa, 28 kDa).

Specification

• Cat. No.: TPMT-76H
• Description: TPMT, thiopurine S-methyltransferase, is a cytosolic enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine and azathioprine are used as chemotherapeutic agents. TPMT activity exhibits autosomal codominant genetic polymorphism, and patients inheriting TPMT-deficiency are at high risk of potentially fatal hematopoietic toxicity.
• Source: Escherichia Coli.
• Formulation: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 0.2 mM PMSF, 2 mM EDTA.
• Molecular Weight: 28 kDa (245 aa).
• Purity: > 95% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Amino acid Sequence: MDGTRTSLDI EEYSDTEVQK NQVLTLEEWQ DKWVNGKTAF HQEQGHQLLK KHLDTFLKGK SGLRVFFPLC GKAVEMKWFA DRGHSVVGVE ISELGIQEFF TEQNLSYSEE PITEIPGTKV FKSSSGNISL YCCSIFDLPR TNIGKFDMIW DRGALVAINP GDRKCYADTM FSLLGKKFQY LLCVLSYDPT KHPGPPFYVP HAEIERLFGK ICNIRRLEKV DAFEERHKSW GIDCLFEKLY LLTEK
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: TPMT thiopurine S-methyltransferase [ Homo sapiens ]
• Synonyms: TPMT; Thiopurine S-methyltransferase; EC 2.1.1.67; Thiopurine methyltransferase; OTTHUMP00000016076; S-adenosyl-L-methionine:thiopurine S-methyltransferase.
• Gene ID: 7172
• mRNA Refseq: NM_000367
• Protein Refseq: NP_000358
• MIM: 187680
• UniProt ID: P51580
• Chromosome Location: 6p22.3
• Function: methyltransferase activity; thiopurine S-methyltransferase activity; transferase activity.