Recombinant Rattus Clusterin, His-tagged, T7-tagged
Cat.No. : | Clu-108R |
Product Overview : | The Rat Clusterin was constructed as a recombinant protein with N-terminal fusion of T7-Tag (16AA) and C-terminal fusion of His-Tag (9AA). The Clusterin Rat His-Tagged Fusion Protein, produced inE.coli, is 26.5 kDa protein containing 215 amino acid residues of the APO-J Rat and 25 additional amino acid residues – His-Tag, T7-Tag. |
- Specification
- Gene Information
- Related Products
Cat. No. : | Clu-108R |
Description : | Clusterin also named Apoliprotein J (APO-J) is a 75-80 kD disulfide-linked heterodimeric protein containing about 30% of N-linked carbohydrate rich in sialic acid. The precursor polypeptide chain is cleaved proteolytically to remove the 22-mer secretory signal peptide and subsequently between residues 227/228 to generate and the chains. These are assembled in anti-parallel to give a heterodimeric molecule in which the cysteine-rich centers are linked by five disulfide bridges and are flanked by two predicted coiled-coil -helices and three predicted amphipathic a-helices. It is able to bind and form complexes with numerous partners such as immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin and others. |
Source : | Escherichia Coli. |
Amino acid sequence : | MASMTGGQQM GRDPNSSSPF YFWMNGDRID SLLESDRQQS QVLDAMQDSF TRASGIIDTL FQDRFFTHEP QDIHHFSPMG FPHKRPHLLY PKSRLVRSLM PLSHYGPLSF HNMFQPFFDM IHQAQQAMDV QLHSPALQFPDVDFLKEGED DRTVCKEIRH NSTGCLKMKG QCEKCQEILS VDCSTNNPAQ ANLRQELNDS LQVAERLTQQ YNELLHSLQS KMLNTSSLLE QALEHHHHHH |
Specificity : | The amino acid sequence of the Clusterin Rat recombinant is 100% homologous to the amino acid sequence 146-360 of the Clusterin Rat precursor. |
Purification Method : | Ni-NTA chromatography. |
Purity : | Greater than 95% (SDS PAGE analyzed). |
Formulation : | Sterile filtered and lyophilized from 0.5 mg/ml in 0.01M Tris pH 7.2. |
Solubility : | Add 0.2 ml of deionized H2O and let the lyophilized pellet dissolve completely. |
Applications : | Western blotting |
Storage : | Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized protein remains stable until the expiry date when stored at -20°C. |
Gene Name : | Clu clusterin [ Rattus norvegicus ] |
Synonyms : | Clu; Clusterin; CLI; APOJ; SGP2; SGP-2; SP-40; Trpm2; Trpmb; TRPM-2; TRPM2B; RATTRPM2B; CLU; TRPM2; KUB1; AAG4; MGC24903; Dimeric acidic glycoprotein; DAG protein; Testosterone repressed prostate message-2; Sulfated glycoprotein-2; Complement lysis inhibitor;SP-40; MGC24903; Apolipoprotein J |
Gene ID : | 24854 |
mRNA Refseq : | NM_053021 |
Protein Refseq : | NP_444180 |
UniProt ID : | P05371 |
Chromosome Location : | 15p12 |
Function : | protein binding |
Products Types
◆ Recombinant Protein | ||
CLU-13H | Recombinant human CLU protein (23-449aa), N-6×His-tagged | +Inquiry |
CLU-3372C | Recombinant Chinese hamster CLU protein(Met1-Glu447), His-tagged | +Inquiry |
CLU-746R | Recombinant Rhesus Macaque CLU Protein, His (Fc)-Avi-tagged | +Inquiry |
CLU-1526H | Recombinant Human CLU Protein, GST-tagged | +Inquiry |
Clu-040M | Recombinant Mouse Clu Protein, MYC/DDK-tagged | +Inquiry |
◆ Native Protein | ||
CLU-67H | Native Human Clusterin | +Inquiry |
CLU-19H | Native Human Clusterin Protein | +Inquiry |
◆ Lysates | ||
CLU-2195MCL | Recombinant Mouse CLU cell lysate | +Inquiry |
CLU-2198HCL | Recombinant Human CLU cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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