Recombinant Human STX1A

• Cat.No.: STX1A-214H
• Product Overview: Recombinant syntaxin 1A protein(1-226aa) contains N-terminal domain(Habc) and t_SNARE domain(H3 domain) and this protein was overexpressed in E.coli and purified by using the conventional column chromatography techniques.

Specification

• Description: This gene encodes a member of the syntaxin superfamily. Syntaxins are nervous system-specific proteins implicated in the docking of synaptic vesicles with the presynaptic plasma membrane. Syntaxins possess a single C-terminal transmembrane domain, a SNARE [Soluble NSF (N-ethylmaleimide-sensitive fusion protein)-Attachment protein REceptor] domain (known as H3), and an N-terminal regulatory domain (Habc). Syntaxins bind synaptotagmin in a calcium-dependent fashion and interact with voltage dependent calcium and potassium channels via the C-terminal H3 domain. This gene product is a key molecule in ion channel regulation and synaptic exocytosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Source: E.coli
• Species: Human
• Form: In 20mM Tris (pH 7.5), 1mM DTT, 10% glycerol
• Molecular Mass: 26.1 kDa (226 aa), confirmed by MALDI-TOF.
• AA Sequence: MKDRTQELRT AKDSDDDDDV AVTVDRDRFM DEFFEQVEEI RGFIDKIAEN VEEVKRKHSA ILASPNPDEK TKEELEELMS DIKKTANKVR SKLKSIEQSI EQEEGLNRSS ADLRIRKTQH STLSRKFVEV MSEYNATQSD YRERCKGRIQ RQLEITGRTT TSEELEDMLE SGNPAIFASG IIMDSSISKQ ALSEIETRHS EIIKLENSIR ELHDMFMDMA MLVESQ
• Purity: > 95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: STX1A syntaxin 1A (brain) [ Homo sapiens (human) ]
• Official Symbol: STX1A
• Synonyms: STX1A; syntaxin 1A (brain); STX1; HPC-1; p35-1; STX1A; Syntaxin-1A; Neuron-specific antigen HPC-1
• Gene ID: 6804
• mRNA Refseq: NM_004603
• Protein Refseq: NP_004594
• MIM: 186590
• UniProt ID: Q16623
• Chromosome Location: 7q11.23
• Pathway: Acetylcholine Neurotransmitter Release Cycle; Dopamine Neurotransmitter Release Cycle; GABA synthesis, release, reuptake and degradation
• Function: ATP-dependent protein binding; SNAP receptor activity; calcium channel inhibitor activity