Recombinant Human SNRNP70, His-tagged
Cat.No. : | SNRNP70-227 |
Product Overview : | Recombinant Human U1-snRNP 68kDa (His-tagged) cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein (lacking 66 internal amino acids outside the known epitope-containing areas). The Calculated molecular weight is 44.8 kDa (U1-snRNP 68) displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight and the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis). |
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Cat. No. : | SNRNP70-227H |
Description : | Small nuclear ribonucleoprotein complexes (abbreviated as U-snRNP) are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus and consists of one small uridylate-rich RNA (U1 RNA) complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins (B/B", D1, D2, D3, E, F, G) form a core subparticle that is common to all U-snRNP complexes. Both the U1-specific proteins and the Sm core particle are targets of autoantibodies which classically have been called the RNP and RNP-Sm specificities, respectively. A clean diagnostic distinction of these specificities has been complicated by the biochemical difficulties of producing clean subparticle fractions from native sources. The use of single recombinant proteins as antigenic targets guarantees a much higher sensitivity and specificity and is the only way to determine RNP antibodies sensu stricto without the disturbing influence of Sm antigens; also with single U1 proteins antibodies will be detected which can be missed because of steric hindrance when using the RNP-Sm complex in an assay. Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease (MCTD) and 30% of patients with SLE. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients. The 68/70 kDa nomenclature of this protein refers to the fact that different splice variants of the protein are found in human cells. |
Source : | E.Coli. |
Formulation : | Recombinant Human U1-snRNP is supplied at a of 0.9mg/ml in16mM HEPES buffer pH-8, 250mM sodium chloride, and 20% glycerol. |
Immunological Functions : | 1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera). |
Applications : | Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera (Mixed Connective Tissue Disease). |
Coating Concentration : | 0.3-0.6 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination. |
Also Avialable : | U1 snRNP A, U1 snRNP B/B, U1 snRNP C |
Purity : | Greater than 90% (SDS-PAGE). |
Storage Conditions : | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°Cfor longer periods of time. Avoid multiple freeze-thaw cycles. |
Gene Name : | SNRNP70 small nuclear ribonucleoprotein 70kDa (U1) [ Homo sapiens ] |
Synonyms : | SNRNP70; small nuclear ribonucleoprotein 70kDa (U1); RPU1; U1AP; U170K; U1RNP; RNPU1Z; SNRP70; U1-70K; U1 small nuclear ribonucleoprotein 70 kDa; small nuclear ribonucleoprotein 70kDa (RNP antigen); U1AP1; U1RNP;snRNP70; U1 snRNP 70 kDa; small nuclear ribonucleoprotein 70kD polypeptide (RNP antigen); small nuclear ribonucleoprotein 70kDa; small nuclear ribonucleoprotein 70kDa polypeptide (RNP antigen) |
Gene ID : | 6625 |
mRNA Refseq : | NM_003089 |
Protein Refseq : | NP_003080 |
MIM : | 180740 |
UniProt ID : | P08621 |
Chromosome Location : | 19q13.3 |
Pathway : | Influenza Infection; Gene Expression |
Function : | RNA binding; nucleotide binding; protein binding |
Products Types
◆ Recombinant Protein | ||
SNRNP70-2711H | Recombinant Human SNRNP70 Protein, His-tagged | +Inquiry |
SNRNP70-4190R | Recombinant Rhesus Macaque SNRNP70 Protein, His (Fc)-Avi-tagged | +Inquiry |
SNRNP70-2059H | Recombinant Human SNRNP70 Protein, His (Fc)-Avi-tagged | +Inquiry |
Snrnp70-6003M | Recombinant Mouse Snrnp70 Protein, Myc/DDK-tagged | +Inquiry |
SNRNP70-227H | Recombinant Human SNRNP70 protein | +Inquiry |
◆ Lysates | ||
SNRNP70-1620HCL | Recombinant Human SNRNP70 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionAutoantibodies against SNRNP70 are frequently found in individuals with autoimmune diseases, serving as a diagnostic marker for conditions like SLE.
Common methods include immunoprecipitation, enzyme-linked immunosorbent assay (ELISA), and Western blotting.
Monitoring anti-SNRNP70 antibody levels during treatment may offer insights into the effectiveness of therapeutic interventions in autoimmune diseases.
Understanding the role of SNRNP70 in autoimmune diseases could lead to the development of targeted therapies aimed at modulating its activity.
Yes, anti-SNRNP70 antibodies are also associated with other autoimmune diseases such as mixed connective tissue disease (MCTD) and rheumatoid arthritis.
Customer Reviews (3)
Write a reviewThis may include providing detailed protocols, troubleshooting advice, and suggestions for experimental design.
The manufacturer can provide researchers with high-quality ARMC6 protein, ensuring it is properly purified, characterized, and quality-controlled.
This collaboration may extend beyond the purchase of the SNRNP70 protein, potentially leading to additional support, joint research projects, or access to expertise in the field.
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