| Description : |
This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. |
| Source : |
E.coli |
| Species : |
Human |
| Form : |
Lyophilized from a 0.2μm filtered solution in 30 % Acetonitrile and 0.1 % TFA. |
| Molecular Mass : |
Approximately 53.5 kDa, a single non-glycosylated polypeptide chain containing 465 amino acids. |
| Protein length : |
465 |
| AA Sequence : |
STRSVSSSSYRRMFGGPGTASRPSS SRSYVTTSTRTYSLGSALRPSTSRS LYASSPGGVYATRSSAVRLRSSVPG VRLLQDSVDFSLADAINTEFKNTRT NEKVELQELNDRFANYIDKVRFLEQ QNKILLAELEQLKGQGKSRLGDLYE EEMRELRRQVDQLTNDKARVEVERD NLAEDIMRLREKLQEEMLQREEAEN TLQSFRQDVDNASLARLDLERKVES LQEEIAFLKKLHEEEIQELQAQIQE QHVQIDVDVSKPDLTAALRDVRQQY ESVAAKNLQEAEEWYKSKFADLSEA ANRNNDALRQAKQESTEYRRQVQSL TCEVDALKGTNESLERQMREMEENF AVEAANYQDTIGRLQDEIQNMKEEM ARHLREYQDLLNVKMALDIEIATYR KLLEGEESRISLPLPNFSSLNLRET NLDSLPLVDTHSKRTLLIKTVETRD GQVINETSQHHDDLE |
| Endotoxin : |
Less than 0.1 EU/µg of rHuVimentin as determined by LAL method. |
| Purity : |
>95% by SDS-PAGE and HPLC analysis. |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
| Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4 mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
